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identifiers mids  (New England Biolabs)


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    New England Biolabs identifiers mids
    Identifiers Mids, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 2555 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/identifiers+mids/NEBNext+Multiplex+Oligos+for+Illumina/pm39853912-191-18-21
    Average 94 stars, based on 2555 article reviews
    identifiers mids - by Bioz Stars, 2026-09
    94/100 stars

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    Multiplex Assay:

    Article Title: rDNA Copy Number Variation and Methylation During Normal and Premature Aging.
    Article Snippet: PCR products were cleaned with Agencourt AMPure XP beads (Beckmann Coulter, Krefeld, Germany), quantified using the Qubit dsDNA BR Assay system kit (Invitrogen, Karlsruhe, Germany), and diluted to a concentration of 0.2 ng/μL. .. In the second PCRs, the two different amplicons for each sample were pooled together and barcoded with multiple identifiers (MIDs), using NEBNext Multiplex Oligos for Illumina (Dual Index Primers Set 1 and 2). ..



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    Samples used as positive controls to validate the deep amplicon sequencing approach for the accurate detection of species composition.

    Journal: Frontiers in Veterinary Science

    Article Title: Development of a novel Haemabiome tool for the high-throughput analysis of haemopathogen species co-infections in African livestock

    doi: 10.3389/fvets.2024.1491828

    Figure Lengend Snippet: Samples used as positive controls to validate the deep amplicon sequencing approach for the accurate detection of species composition.

    Article Snippet: The second round of amplification was performed to anneal the Nextera II multiplex identifier tags (MID) and sequencing primers ( ).

    Techniques: Amplification, Sequencing, Cell Culture

    Phylogenetic tree of Anaplasma and Ehrlichia spp. determined in the subset of 31 IDEAL animals. Relationships were inferred from phylogenetic analysis of sequence data for a ~132-bp region of the 16S rRNA gene by Bayesian inference. Sequences obtained in this study are highlighted in bold with the number of samples with a specific pathogen sequence identified, indicated in brackets. Numbers at the nodes indicate % bootstrap support and the scale bar represents 0.1 substitutions per site.

    Journal: Frontiers in Veterinary Science

    Article Title: Development of a novel Haemabiome tool for the high-throughput analysis of haemopathogen species co-infections in African livestock

    doi: 10.3389/fvets.2024.1491828

    Figure Lengend Snippet: Phylogenetic tree of Anaplasma and Ehrlichia spp. determined in the subset of 31 IDEAL animals. Relationships were inferred from phylogenetic analysis of sequence data for a ~132-bp region of the 16S rRNA gene by Bayesian inference. Sequences obtained in this study are highlighted in bold with the number of samples with a specific pathogen sequence identified, indicated in brackets. Numbers at the nodes indicate % bootstrap support and the scale bar represents 0.1 substitutions per site.

    Article Snippet: The second round of amplification was performed to anneal the Nextera II multiplex identifier tags (MID) and sequencing primers ( ).

    Techniques: Sequencing

    Phylogenetic relationship among consensus sequences of Theileria and Babesia. spp. determined in IDEAL subset animals. Relationships were inferred from phylogenetic analysis of sequence data for a ~405-bp region of the 18S rRNA gene by Bayesian inference. Sequences obtained in this study are highlighted in bold with the number of samples with specific pathogen sequence identified indicated in brackets. Numbers at the nodes indicate % bootstrap support and the scale bar represents 0.1 substitutions per site.

    Journal: Frontiers in Veterinary Science

    Article Title: Development of a novel Haemabiome tool for the high-throughput analysis of haemopathogen species co-infections in African livestock

    doi: 10.3389/fvets.2024.1491828

    Figure Lengend Snippet: Phylogenetic relationship among consensus sequences of Theileria and Babesia. spp. determined in IDEAL subset animals. Relationships were inferred from phylogenetic analysis of sequence data for a ~405-bp region of the 18S rRNA gene by Bayesian inference. Sequences obtained in this study are highlighted in bold with the number of samples with specific pathogen sequence identified indicated in brackets. Numbers at the nodes indicate % bootstrap support and the scale bar represents 0.1 substitutions per site.

    Article Snippet: The second round of amplification was performed to anneal the Nextera II multiplex identifier tags (MID) and sequencing primers ( ).

    Techniques: Sequencing